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SOLVED: Your bacterial transformation worked and you see 20 colonies! Using the transformation efficiency equation below, calculate the transformation efficiency if 15 μl of plasmid DNA was used. The plasmid DNA concentration
SOLVED: Transformation efficiency is the quantitative determination of the number of cells transformed per pg of plasmid DNA. In essence, it is an indicator of the success of the transformation experiment. You
HYPOTHESIS:
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SOLVED: Calculate the transformation efficiency for the experimental results in the table below. For your calculations, assume that 10 μL of plasmid was used in the experiment and the plasmid concentration was
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SOLVED: I would like to understand how to solve this example step-by-step to prepare for an exam: 4.B. Example of Transformation Efficiency Calculation After 100 μl of competent cells are transformed with
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